NGS services cover consulting, library preparation, sequencing, and analysis for your research. The earlier we can be involved in your experimental design, the better result we can deliver to you. Our current services include the following applications:
| Category | Application | Purpose | ||
|---|---|---|---|---|
| Genomics | Whole Genome Sequencing (WGS) | Whole genome sequencing (WGS), also known as full genome sequencing, complete genome sequencing, or entire genome sequencing, is the process of determining the entirety, or nearly the entirety, of the DNA sequence of an organism's genome at a single time. | ||
| Whole Exome Sequencing (WES) | Exome sequencing, also known as whole exome sequencing (WES), is a genomic technique for sequencing all of the protein-coding regions of genes in a genome (known as the exome). The goal of this approach is to identify genetic variants that alter protein sequences, and to do this at a much lower cost than whole-genome sequencing. | |||
| De novo Sequencing | De novo sequencing refers to sequencing a novel genome where there is no reference sequence available for alignment. | |||
| Targeted Sequencing | Targeted sequencing uses deep sequencing to detect known and novel variants within your region of interest. This method generally requires less sample input and produces a smaller amount of data than WGS, making analyses more manageable. | |||
| Transcriptomics | total RNA-Seq | Total RNA-Seq offers the most comprehensive wholetranscriptome analysis. | ||
| mRNA-Seq | mRNA-Seq is ideal if the research is focused only on the coding region and limited amounts of starting material are available. | |||
| 3’mRNA-Seq | 3'mRNA-seq provides reliable gene expression quantification at a minimum cost. Suitable for large number of samples. | |||
| miRNA-Seq | MicroRNA sequencing (miRNA-seq) sequences microRNAs, also called miRNAs. miRNA-seq differs from other forms of RNA-seq in that input material is often enriched for small RNAs. | |||
| Epigenetics | ChIP-Seq | ChIP-Seq identifies the binding sites of DNA-associated proteins and can be used to map global binding sites for a given protein. ChIP-Seq typically starts with crosslinking of DNA-protein complexes. | ||
| ATAC-Seq | ATAC-seq identifies accessible DNA regions by probing open chromatin with hyperactive mutant Tn5 Transposase that inserts sequencing adapters into open regions of the genome. | |||
| single-cell | scRNA-Seq | Single-Cell RNA-Seq provides transcriptional profiling of thousands of individual cells. This level of throughput analysis enables researchers to understand at the single-cell level what genes are expressed, in what quantities, and how they differ across thousands of cells within a heterogeneous sample. |
General notes for all applications:
- Experimental design
- Please make sure that you have biological replicates or technical replicates. The statistical analyses won’t work without replicates.
- Please clearly define the comparison you want to perform. The basic analysis includes only one comparison.
- Samples
- Please prepare an overview of the samples according to our template.
- Please see this page for some guidelines of sample names.
- Please check the request form for the required information about your samples.