If you are a very frequent user you can receive an in depth introduction to the machine after which you can use it yourself.
The largest capillary of the CellenOne has an opening diameter of 100µm. In principle, any cell with a smaller diameter can be used for isolation. The recommendations are sizes of 0.5 µm – 80 µm.
The stability of the CellenONE drop is highly dependent on the liquid composition. Media or buffer which are recommended by the manufacturer are for example PBS (preferred), DMEM, RPMI, Hams’s F12.
The CellenOne has four fluorescent channels: blue, green, orange and red. You may for example use Cell Tracker Green and dye only living cells by which you narrow down your isolation conditions. You can find more information about excitation and emission wave lengths of CellenONE by following this link.
The CellenOne can dispense cells in individual vials (1 Isolation per vial), standard glass slides, and differently sized plates. You can use multiple plates. Theoretically you can sort as many cells as there are present in your sample. Please contact the Genomics Facility to find out more about plate and vial compatibilities.
It depends on the cell density of your sample. Cell isolation with the CellenONE works best with a cell density of 100-200 cells/µl. We need a total volume of minimum 100 µl up to 1000 µl to process your cells; preferably delivered in an Eppendorf tube on ice.
You can find out more about the CellenONE by following this link.
You can download the template for the sample sheet here. Please be as precise as possible about the samples you will submit.
Please feel free to let us know your questions and problems so that we can improve this page by adding necessary clarification here.
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